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Engineer / Master in protein biochemistry

Engineer / Master in protein biochemistry

Universite de LorraineQueensland, Australia
6 days ago
Job description

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Position Overview

We are seeking a highly motivated engineer or Master’s graduate to join the THIONICO project, as part of the interdisciplinary ARTEMIS program.

The goal of THIONICO is to biochemically and structurally characterize atypical poplar thioredoxins of unknown function that form oligomers in solution. This work will contribute to extend our understanding of the redox metabolism in plants.

The selected candidate will have to play a central role contributing to the development of the expression of protein oligomers in the plant-based eukaryotic system Nicotiana benthamiana and will participate in both their biochemical and structural characterization.

Responsibilities

In compliance with protocols available in the literature and laboratory and a rigorous quality approach, your tasks will include :

  • Building constructs using modular cloning.
  • Developing the N. benthamiana expression system for recombinant proteins.
  • Investigating the biochemical activities and properties of the obtained protein complexes.
  • Preparing samples for the structural analysis of the complexes.
  • Contributing to the broader ARTEMIS operational challenge OC1 - Decipher the biology of the tree-microbiome systems.

Candidate Profile

We are looking for a highly motivated candidate with a Master’s degree (or equivalent) in biochemistry, structural biology, plant sciences or related fields and proven experience in one or several of the following aspects :

  • Recombinant protein expression and purification
  • Protein complex characterization, enzymology, redox biology or structural biology
  • Solid skills in molecular biology techniques particularly Golden Gate cloning) and transient expression in N. benthamiana- Experience with protein expression in eukaryotic system is a strong plus
  • Excellent organizational skills and the ability to work effectively in a collaborative and interdisciplinary environment
  • Location : Vandœuvre-lès-Nancy (Faculty of Sciences and Technology)
  • Salary : According to current pay scale, between 2200 and 2400 € gross salary
  • Application

    Please send your CV, transcript of records, motivation letter and recommendation letters or the name of referent persons to :

    linda.de-bont@univ-lorraine.fr and nicolas.rouhier@univ-lorraine.fr

    Seniority level

    Seniority level

    Entry level

    Employment type

    Employment type

    Full-time

    Job function

    Job function

    Engineering and Information Technology

    Industries

    Higher Education

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    Protein Biochemistry • Queensland, Australia

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